Assessment of pathogenicity of resting spores of Plasmodiophora brassicae in soil by fluorescence microscopy.

Abstract

A fluorescence microscopic method was applied to assess directly the pathogenicity of resting spores of Plasmodiophora brassicae in soil. Infested soils were stained with a mixture solution of two fluorochromes, calcofluor white M2R and ethidium bromide, and were observed by fluorescence microscopy (phase contrast, UV filter set, oil immersion).