Adenovirus-infected, Cell-specific, DNA-binding Proteins

Abstract

The human adenoviruses contain a genome that could theoretically code for 25–50 average size proteins. Of this total, approximately ten polypeptides have been detected in the mature virion (Maizel et al. 1968; Everitt et al. 1973) and these could account for up to 35–40% of this coding capacity. Productive infection of human cells with types 2 or 5 adenovirus leads to the appearance of several infected cell-specific proteins not found in the mature virion and detected by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (Russell and Skehel 1972; Anderson et al. 1973; Walter and Maizel 1974). Some of these proteins are precursors of adenovirus virion polypeptides (Anderson et al. 1973), while others appear to be synthesized at early times after infection, before viral DNA synthesis begins. At least three early adenovirus-induced proteins (MW 71,000, 19,000 and 10,000) have been described in this manner (Walter and Maizel 1974;...