Demonstration of Eaton's Agent in Tissue Culture.

Abstract

The Eaton agent was localized in secondary monkey kidney cell cultures by means of fluorescent antibody staining employing either human atypical pneumonia convalescent sera or rabbit antiserum. Morphologic study of material stained by the Giemsa method revealed the occurrence of extracellular growths resembling colonies with an average diameter of 10 micra. These findings plus other characteristics of the agent summarized suggest an intimate relationship to organisms of the genus Mycoplasma.