Optimization of production and partial purification of laccase by Phanerochaete chrysosporium using submerged fermenation

Abstract

Laccases are multi copper oxidases having wide substrate specificity mainly found in white-rot fungi, which are the only microorganisms able to degrade the whole wood components. In contrast to most enzymes, which are generally very substrate specific, laccases act on a surprisingly broad range of substrates, including diphenols, polyphenols, different substituted phenols, diamines, aromatic amines, benzenethiols and even some inorganic compounds such as iodine. As they are capable of degrading a wide variety of compounds they are commercially very significant. This project aims at studying the production optimization of laccase using different carbon sources.