Studies on Metabolism of 2-Methyl-3-o-tolyl-4 (3H)-quinazolinone. I. The Estimation of 2-Methyl-3-o-tolyl-4 (3H)-quinazolinone in Biological Materials.

Abstract

Two methods are described for the estimation of 2-methyl-3-o-tolyl-4 (3H)-quinazolinone (MTQ) in biological fluids and tissues. In the first, MTQ is extracted from alkalinized biological material with hexane, the solvent is evaporated, and the residue is dissolved in dilute hydrochloric acid, and measured spectrophotometrically at 234mμ. The second, suitable for tissues, is the method in which normal biological blank becomes negligible by geometrical treatment of its absorbances at three wave lengths. These procedures permit the determination of MTQ in amounts as 1 μg./cc. of final measurement solution. The methods are specific for MTQ in that they do not include any transformation products of the compound.