Optimization of the EMIT Immunoassay Procedure for the Analysis of Cannabinoids in Methanolic Blood Extracts

Abstract

EMIT assays are commonly used for the detection of cannabinoids in urine and have been widely adapted to accommodate blood samples. Over recent years, several different kits have been produced by the assay manufacturer for this purpose. The newest kit was found to be significantly less sensitive for methanolic blood extracts than kits previously available. Modifications are described to improve the sensitivity of the newer cannabinoid kit as applied to blood extracts. In addition, information is presented which demonstrates enhanced performance of this assay procedure by the use of a commercially available enzyme substrate supplement.