Specific Determination of Proline in Biological Materials.

Abstract

A specific method for determining proline is reported, based on treatment of isatin-developed paper chromatograms with dilute acid. None of the 20 other amino acids of synthetic medium M 150, including hy-droxyproline, reacted by this procedure. The proline chromogen exhibits an absorption maximum at 600 mμ. The color is remarkably stable and can be used for the quantitative determination of proline over the range of 1 to 16 μg.