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Separation of Lymphocytes in Human Blood by Means of Glass Wool Column.
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1959
Year
Laboratory HematologyGranulocyteBioanalysisImmunologyHematologyLymphatic SystemBlood CellDifferential AdsorptionWhole BloodHuman BloodBiomedical EngineeringClinical ChemistryMedicineGlass Wool ColumnChromatography
The method of differential adsorption has been successfully applied to separation of lymphocytes from granulocytes in human blood. When whole blood was passed through a short column of siliconized glass wool, the granulocytes were almost quantitatively retained on the column, and uninjured lymphocytes in 30-89% yield appeared in the effluent blood.