A Rapid Radioimmunoassay Method of Growth Hormone in Dog Plasma

Abstract

A rapid and sensitive homologous radioimmunoassay (RIA) system is described for the measurement of growth hormone (GH) in dog plasma. The method requires only 40 hr and is able to detect concentrations of GH as low as 1.0 ng/ml of dog plasma. Antiserum to canine growth hormone (cGH) prepared in monkeys, exhibited a complete cross-reactivity with porcine GH, suggesting that the latter can substitute cGH in a heterologous radioimmunoassay for cGH. Studies on GH regulation performed with this RIA in the unanesthetized dog showed that this species resembles the primate more than the rat or the rabbit.