Development and Validation of a Simple UV Spectrophotometric Method for the Determination of Cefotaxime Sodium in Bulk And Pharmaceutical Formulation

Abstract

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefotaxime sodium in bulk and pharmaceutical formulation. The solvent used throughout the experiment was methanol and water. Absorption maximum ( max ) of the drug was found to be 260 nm. The quantitative determination of the drug was carried out at 260 nm and Beer's law was obeyed in the range of 10-30g/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.025x + 0.0028 with correlation coefficient R 2 of 0.9995. The recovery values for cefotaxime sodium ranged from 99.95% -100.21%.The percent relative standard deviation (RSD %) of interday precision range was 0.099 -0.140% and intraday precision range was 0.098 -0.132%. The limit of detection and limit of quantification was 0.079g/mL and 0.154g/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.142 -0.221%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.