Radioimmunoassay for Rat Luteinizing Hormone with Antiovine LH Serum and Ovine LH-131I

Abstract

SummaryA highly sensitive and specific radioimmunoassay for rat LH has been developed utilizing antiovine LH serum and ovine LH for iodination. Estimates of the LH potency of pituitary preparations with widely varying LH to FSH and LH to TSH potencies obtained with this assay agree with estimates obtained with bioassay and with those obtained with a radioimmunoassay utilizing antirat LH serum and rat LH for iodination. With this technique it has been possible to measure the LH in serum obtained from females throughout the estrus cycle and in serum from normal and castrated males.