Antibiogram and beta-lactamase production of Staphylococcus aureus isolates from different human clinical specimens in a tertiary health institution in Ile-ife, Nigeria.

Abstract

The prevalence of s-lactamase producing Staphylococcus aureus infections and their antimicrobial susceptibility pattern are reported in this study. Beta-lactamase production was detected using a standard starch paper technique on all the isolates. And In Vitro antimicrobial susceptibility study was conducted by agar disc diffusion method. One hundred S.aureus isolates obtained from different clinical specimens were studied. Out of total 100 strains of S. aureus, 80% were found to be s-lactamase producer, which probably accounted for 100% and 96% resistant rate obtained for penicillin and ampicillin respectively.Among the s-Lactamase producing organisms, susceptibility to antibiotics were: erythromycin (82.5%), Cephalexin (71%) Ceftriaxone (70%), Cloxacillin (66%), Others were chloramphenicol, Gentamicin, tetracycline and streptomycin with 62.5%, 61%, 30% and 53.8% susceptible respectively. This thus suggests that clinicians should enlighten patients on the consequences of indiscriminate use of Penicillin and other antimicrobial agents.