Simultaneous Determination of Five Volatile and Non‐Volatile N‐Nitrosamines in Biological Fluids and Cosmetic Products by Liquid Chromatography with Photodiode Array Detection

Abstract

Abstract An HPLC method has been developed for the determination of nitrosamines. Five nitrosamines were separated simultaneously on a 250 mm×4.6 mm i.d. Phenomenex Luna CN (particle size 5 µm) column with methanol‐1.0 m M K2HPO4 (pH 4.0) solvent, programmed as mobile phase and with a photodiode array detector. The limits of detection were 0.02, 0.02, 0.02, 0.03, and 0.03 mg L−1 for N‐nitrosodiethanolamine, N‐nitroso‐bis‐(2‐hydroxypropyl)amine, N‐nitrosodimethyamine, N‐nitrosodi‐n‐propylamine and N‐nitrosodiphenylamine, respectively. The method is applied for the simultaneous quantitative determination of nitrosamine in cosmetics and biological fluids.