Experimental Biology and Medicine · 1949 · 74 citations · 0 references
Life SciencesBiochemistryEndocrinologyHormone BiochemistryReproductive EndocrinologySteroid BiochemistryGonadal HormonesGonadal Steroid HormonesGonadal Hormone AssaysHydroxide PrecipitationReproductive Hormone AssaysUrinary Hormone MeasurementUrinary Steroid Analysis
A method is described for the adsorption of gonadotrophic hormones from urine onto kaolin and their subsequent elution with ammonium hydroxide. The relatively small volume of the eluate reduces the amount of alcohol needed to precipitate the hormone from solution, the final extracts are less toxic and can be prepared in a shorter time. The hormone assays are comparable to those obtained with the usual alcohol recipitation method.