Liberation of Histamine and Heparin by Peptone from the Isolated Dog's Liver.

Abstract

Experiments on the perfusion of dog's liver show that the presence of blood in the perfusing fluid is important for the production of an appreciable discharge of histamine and heparin by peptone. The best results were obtained when anticoagulants were excluded and the blood was preserved from clotting in silicone-treated receptacles. When Tyrode's solution was used as a vehicle for the perfusion, only small amounts of histamine and traces of heparin appeared in the perfusates. The experiments presented in this paper do not afford any direct proof but are suggestive of the participation of platelets and possibly also of leucocytes in the mechanism of the discharge of histamine and heparin from the liver.