Spontaneous Formation of Vesicles by Sodium 2-Dodecylnicotinate in Water

Abstract

The surface activity and aggregation behavior of a synthesized nicotinic acid based anionic surfactant, sodium 2-dodecylnicotinate, were studied in aqueous solution. The self-assembly formation was investigated by use of a number of techniques, including surface tension and conductivity measurements, fluorescence spectroscopy, dynamic light scattering measurement, gel permeation chromatography, and microscopy. The amphiphile exhibits two breaks in the surface tension vs concentration plot, indicating stepwise aggregate formation and thus producing two values of the aggregation concentration. Stepwise aggregation of the amphiphile was further confirmed by steady-state fluorescence spectroscopy using pyrene as a probe molecule, and also the micropolarity of the aggregates was determined. The rigidity of the microenvironment was estimated by determining steady-state fluorescence anisotropy using 1,6-diphenyl-1,3,5-hexatriene as a fluorescence probe molecule. The average hydrodynamic radius and size distribution of the aggregate suggest formation of larger aggregates in aqueous solution. The formation of vesicles in water was established by conductivity measurement and a dye entrapment experiment. The entrapment of a small solute and the release capability have also been examined to demonstrate these bilayers form enclosed vesicles. Transmission electron micrographs revealed the existence of closed vesicles and closed tubules in aqueous solution. Therefore, for the first time, it has been observed that this simple single-chain nicotinic acid based amphiphile spontaneously assembles to vesicles in aqueous solution.