Selectivity in Integration Sites of Adenoviral DNA

Abstract

We have set out to study the sites of viral DNA integration in two systems: (1) adenovirus 12 (Ad12)transformed hamster and rat cells and (2) human cells permissively infected with human adenovirus 2 (Ad2). It has been shown by a combination of methods that adenoviral DNA and cellular DNA can become covalently linked both in Ad12-transformed hamster and rat cells (Groneberg and Doerfler 1976; Groneberg et al. 1977; for review, see Doerfler et al. 1977; Sutter et al. 1978; Ibelgaufts et al. 1980) and in Ad2-infected and Ad5-infected human cells (Burger and Doerfler 1974; Baczko et al. 1978; Tyndall et al. 1978). One of the striking features emerging from these investigations is the limited number of sites at which viral DNA is inserted into the host DNA, even though a large number of viral DNA copies have been shown to be integrated in the transformed cells that we have studied...