Design and Analysis of Two Phase Experiments

Abstract

It sometimes happens in experimental work that the effects of different treatments cannot be measured directly and a further stage of testing is required in order to evaluate them. Examples of this type of situation are studies of the effect of conditions of growth of parent material on resistance to disease or productivity of progeny; the survival of nodule bacteria under various conditions of storage and appraised by inoculating appropriate legume seedlings; and the effect of various treatments on virus multiplication in leaf tissue, the concentration of virus being ascertained by lesion counts on indicator plants.