Antiviral action of<i>Harpulia Cupanioides</i>and<i>Mirabilis Jalapa</i>against tomato spotted wilt virus (TSWV) infecting tomato

Abstract

Mirabilis jalapa and Harpulia cupanioides remain as an unexploited potential to be tapped, as sources of antiviral principles. The antiviral protein from M. jalapa (MAP) and H. cupanioides (HAP) were highly effective in inhibiting TSWV at 60 and 80% saturation. A minimum concentration of 400 μg/ml of MAP was sufficient to inhibit TSWV. HAP at 800 μg/ml recorded 98.41% inhibition of lesion formation. The plants treated with M. jalapa and H. cupanioides challenged with TSWV did not show significant variation in absorbance values when compared to uninoculated healthy plants, indicating the absence of detectable virus through indirect DAS ELISA thus indicating the effectiveness of AVPs. Pre-application of MAP and HAP induced the activity of phenols, PO, PPO and PAL leading to the suppression of TSWV on local lesion and systemic host. Keywords: Mirabilis JalapaHarpulia CupanioidesTswvPoPpoPal