Stem Cell Research · 2016 · 24 citations · 5 references
Life SciencesCell BiologyGeneticsCytogeneticsEmbryogenesisStem Cell BiologyCell Line DevelopmentEmbryonic Stem CellsPluripotent Stem CellsStem Cell EthicsDonor Tissue ProcurementEmbryonic Stem Cell CharacterizationEmbryonic Stem Cell Quality ControlEthical Informed ConsentGood Manufacturing Practice ComplianceHuman Embryonic Stem CellsStem Cell Line Derivation EthicsStem Cell-derived Cell Lines
The human embryonic stem cell line RCe015-A (RC-11) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a fragmented cleavage stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe015-A (RC-11) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro and in vivo. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
5