Propagation of Poliovirus in Chick Embryo Cell Cultures. I. Cultivation of 3 Virus Types

Abstract

Cell cultures were prepared from the chorioallantoic membrane of chick embryos. After 5 transfers these were tested for susceptibility to infection by representative virulent strains of the 3 immunologic types of poliovirus. Multiplication was observed until the series were terminated after 10 or 20 passages. Neutralization tests confirmed the identity of the viruses after passage. The susceptibility of these cells to infection with poliovirus is in marked contrast to that of the tissues from which they were derived.