Publication | Closed Access
Distribution and Valence State of Radiochromium in Intracellularly Labeled Ehrlich Mouse Ascites Carcinoma Cells.
28
Citations
0
References
1958
Year
Chemoprevention StrategyCarcinoma CellsChemical BiologyCancer BiologyRedox BiologyTumor BiologyOxidative StressCancer Cell BiologyRadiopharmaceutical TherapyValence StateRadiation OncologyCancer ResearchBiochemistryOncogenic AgentCell BiologyMalignant DiseaseTotal Intracellular RadioactivityHexavalent ChromiumNatural SciencesRadioanalytical ChemistryCellular BiochemistryMedicine
1. Ehrlich mouse ascites carcinoma cells were labeled with radiochromate (Na2Cr51O4), and the intracellular distribution and valence state of radiochromium studied. 2. Of the total intracellular radioactivity, 71% was associated with the soluble cytoplasmic fraction; of radioactivity of this fraction, ethanol precipitated 52% with the proteins, and 46% remained in the supernate from ethanol precipitation in dialyzable form. 3. Hexavalent chromium (Cr51O4) appeared to be intracellularly reduced to the trivalent state (Cr51+++). 4. The results suggest that the cell membrane of the Ehrlich ascites tumor cell is impermeable to intracellular Cr+++ ions, and that stability of the intracellular label is not dependent on protein binding alone.