The Cultural Conditions Affecting the Mycelial Growth of Grifola umbellata

Abstract

This study was carried out to obtain the basic data for artificial culture of Grifola umbellata. The optimal condition for the mycelial growth was obtained at and pH 4, respectively. G. umbellata showed the most favorable growth on the Hoppkins media. Carbon sources such as glucose, fluctose and manitol were favorable for stimulating a mycelial growth of G. umbellata. Valine, one of nitrogen sources also appeared to be favorable to a mycelial growth. The optimum C/N ratio was about 30:1 in case that 1% glucose as carbon source was mixed with the basal media. Lactic acid as organic acid was most favorable to the mycelial growth. Also, thiamine-Hcl as vitamin source was favorable. The mineral nutrient of or was most favorable to G. umbellata, and its optimal concentration was about 0.01% in and 0.1 % in respectively. Among 4 different cereal extract media, polished rice extract medium which was mixed with silkworm pupae was most suitable for a favorable growth of G. umbellata.