Organ Cultures of Normal, Dysplastic, Hyperplastic, and Neoplastic Human Mammary Tissues<xref ref-type="fn" rid="FN1">2</xref>

Abstract

Explants of 52 human breast biopsy specimens were maintained in organ culture in chemically defined media, both with and without added hormones. In cultures without hormones there was good survival of normal ducts and lobules, various paHerns of mammary dysplasia, ductal carcinoma in situ, fibroadenoma, intraductal papilloma, gynecomastia, and colloid carcinoma; on the other hand, there was poor survival of scirrous carcinoma. Addition of amorphous insulin, aldosterone, and ovine mammotropin, alone or in combination, had no effect on survival and caused no cell proliferation or secretion. At higher concentrations estradiol 17-β sometimes was deleterious for survival.