Plant Regeneration from Callus Cultures Derived from Primordial Leaves of Adult Japanese Persimmon

Abstract

Abstract Primordial leaves of Japanese persimmon ( Diospyros kaki L. cv. Jiro) were excised from dormant buds and cultured on Murashige and Skoog medium with the nitrates reduced to half strength (MS½N) and supplemented with 0.1 to 10.0 µ m zeatin + 0.1 to 10.0 µ m IAA. After 40 days of culture in the dark, yellowish-white calli were formed at 10.0 µ m zeatin + 1.0 µ m IAA. When these calli were transferred to MS½N medium supplmented with zeatin (1.0 to 10.0 µ m ) + IAA (0 to 10.0 µ m ), 24% of them produced adventitious buds at 10.0 µ m zeatin + 0.1 µ m IAA under a 12-hr photoperiod. Further shoot growth occurred on the same medium without IAA. Basal ends of the shoots were quick-dipped in 1.25 m m IBA and placed on half-strength MS½ N medium. Nearly two-thirds of the dipped shoots rooted within 30 days. Plantlets regenerated in vitro were then transferred to a mixture of 1 peat: 1 perlite: 1 vermiculite and acclimatized for potting. Chemical names used: 1 H -indole-3-acetic acid (IAA); 1 H- indole-3-butyric acid (IBA); ( E )-2-methyl-4-(1- H -purin-6-ylamino)-2-buten-1-o1 (zeatin).