Polyhaploid production through anther culture in common wheat

Abstract

Calli were Induced from microspores and polyhaplold seedlings were regenerated from the calli In several common wheats by using chemically defined nutrient media. There appeared to be genotype-medium Interaction because plants of some cultivars responded in vitro better than others. Chromosome number in root tips of regenerated plants showed that they were predominantly polyhaploids (2n = 3x = 21) but few had 14 and one had only 7 chromosomes. Twenty-eight regenerated plants were vigorous but completely sterile unless treated with colchicine. One albino was observed.