The study salt and drought tolerance of Sinorhizobium bacteria to the adaptation to alkaline condition

Abstract

Salinity, alkalinity and drought stress are the major abiotic stresses hindering the productivity of alfalfa (Medicago Sativa) in arid and semi-arid regions. Therefore an ecological survey was conducted to characterize 600 Sinirhizobium strains of diverse geographical origin, isolated from the root nodules of alfalfa (Medicago Sativa). Rhizobial isolates were studied under free living conditions. Shinorhizobium sp. Tolerated yeast extract mannitol broth (YEB) containing 0, 1%, 2.5% and 4.5% salt (NaCl, wt/vol) for up 18 h of incubation at 30 C. Growth of Shinorhizobium sp. at 0 6000 water potential 0, -1, -2 and -3.5 M Pa (with PEG , wt/vol) for up 18 h of incubation at 30 C was 0 identical. The results showed that salinity and drought tolerance among isolates was significantly different. All isolates were grouped in two clusters: sensitive and tolerant based on their growth rate 6000 in YEB media containing different concentrations of NaCl and PEG . To obtain strains resistant to alkaline conditions (high pH range between 7-9) the medium was buffered with AMPD buffer, while for the low pH range between 4 and 5 it was buffered with 25 mM HOMOPIPES A positive correlation was found between the salt tolerance and the adaptation to alkaline pH.