American Journal of Tropical Medicine and Hygiene · 1989 · 176 citations · 0 references
Veterinary MedicineImmunologyInfectious DiseasesDiagnostic ParasitologyImmunology Of Infectious DiseasesParasitologyAntigen Detection AssaysHelminth DiagnosticsHelminth ImmunologyParasite AntigensParasitic Disease DiagnosisSerological DiagnosticsClinical Diagnosis Of Parasitic DiseasesDetection Of Helminth ParasitesDiagnostic AntigensHelminth ImmunodiagnosisParasite Antibody DetectionParasitic Antigen DetectionParasitic SerologySerological Diagnosis Of Parasitic Infections
From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate. This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting less than 1 ng antigen/ml serum. In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.