Epidemiological typing of<i>Neisseria gonorrhoeae</i>and detection of markers associated with antimicrobial resistance directly from urine samples using next generation sequencing

Abstract

We found that sequencing the entire genome of N. gonorrhoeae directly from clinical samples is possible using NGS, and that multiple levels of N. gonorrhoeae typing information can be generated. As NAAT only testing becomes more common, this method could be used to detect both known and novel mutations associated with AMR and to generate genotyping information, supporting AMR and epidemiological surveillance in the absence of culturing.