Isolation and Characteristics of Trichoderma harzianum FJ1 Producing Cellulases and Xylanase

Abstract

Strain FJ1, a filamentous fungus isolated from rotten wood, showed high ability to hydrolyze cellulosic materials. To identify the strain FJ1, ITS sequencing analysis and morphological observation were performed. The strain FJ I was identified as Trichoderma harzianum. The strain produced a large amount of CMCase, xylanase, β-glucosidase, and avicelase. Optimal culture conditions for the production of the enzymes, such as pH, temperature, and inoculation concentration, were initial pH 6.0-7.0, 25-30°C, and 10 4 ea-spores/ml in Mandel's medium, respectively. T harzianum FJ I utilized various cellulosic materials and organic nitrogen sources to produce cellulases and xylanase, and also considerably a crystalline and/or insoluble material like Avicel and rice straw. The highest levels of CMCase and xylanase were 41.2 and 65.6 U/ml in 7 days of cultivation using 2.5% of carbon source (Avicel+CMC) and 0.5% of nitrogen source (peptone), respectively.