Sensitive and label-free T4 polynucleotide kinase/phosphatase detection based on poly(thymine)-templated copper nanoparticles coupled with nicking enzyme-assisted signal amplification

Abstract

A novel label-free biosensor for highly sensitive sensing of the activity as well as inhibition of T4 PNKP based on poly T-templated fluorescent CuNPs and nicking enzyme-assisted signal amplification.