Mammalian Spermatogenesis as a Biologic Dosimeter for Radiation

Abstract

Mouse spermatogenesis was used as an in vivo test system obviously suitable as a biologic dosimeter. Ionizing irradiation induced changes in the frequency distribution of the cellular DNA content of whole testis preparations. These changes can be analysed by flow cytometry. Such measurements deliver information on: (1) an increase of the coefficient of variation of the DNA histograms of some spermatogenic cells, (2) induction of diploid mature sperm, and (3) the dose-depending inactivation of the highly sensitive differentiated spermatogonia. In this model especially No. 3 delivers quantitative information on the cytotoxic action of ionizing irradiation and chemical noxae as well, whereas Nos 1 and 2 may be used as qualitative criteria of mutagenic action of physical and chemical agents. No. 2 obviously being more sensitive than No. 1.