Detection of Cytokinins Using a Gas Chromatograph Equipped with a Sensitive Nitrogen-Phosphorus Detector1

Abstract

Abstract A gas chromatograph equipped with a nitrogen-phosphorus detector was used for quantitation of cytokinins. The described system, utilizing permethylated derivatives, will detect as little as 0.1 pg adenine, isopentenyl adenine, benzylamino purine, and kinetin, and 10 pg zeatin. As with all highly sensitive instrumentation, scrupulous procedures for sample preparation are essential.