Characterization of PGP Traits of a Hexavalent Chromium Resistant<i>Raoultella</i>sp. Isolated from the Rice Field near Industrial Sewage of Burdwan District, WB, India

Abstract

Chromium (Cr) is the most toxic at its hexavalent state. Widespread use of chromium for various anthropogenic activities causing rapid decline of the agricultural productivity is now a major global concern. The purpose of this study was to isolate the plant growth promoting (PGP) chromium-resistant bacteria and characterize it before being applied for bioremediation. A potent Cr-resistant rhizobacterium (CrS2) was isolated from the rice field near an industrial sewage and identified as Raoultella sp. based on 16S rDNA sequence homology with some phenotypic characteristics. The strain exhibited Cr(VI) resistance up to 25 mM and also possesses some important PGP traits. The selected CrS2 strain has varied degrees of resistance to other toxic heavy metals/metalloids like arsenic, cadmium, and lead. The removal capacity of chromium was studied in broth cultures. The appropriate growth media for the strain is peptone yeast glucose media with glucose (0.5%) and peptone (1%) as carbon and nitrogen sources, respectively. The strain removed substantial amount of chromium after media optimization. The chromate reductase (EC.1.6.5.2) activity was constitutive in nature of this strain. Thus, the strain CrS2 may be exploited for bioremediation of Cr(VI) in Cr-contaminated agricultural soil, where it might also enhance plant growth promotion.