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Aldehyde-induced protein modifications in human plasma: protection by glutathione and dihydrolipoic acid.

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References

1994

Year

C. O’Neill, Barry Halliwell, Albert van der Vliet, Paul A. Davis, Lester Packer, H. Tritschler, W. J. Strohman, Tris Rieland, Carroll E. Cross, Abraham Z. Reznick
PubMed

Abstract

Exposure of human plasma to gas phase cigarette smoke (CS) produces a depletion of ascorbic acid, peroxidation of lipids (Frei et al. Biochem J 1991; 277: 133-8), and protein modification (as measured by protein carbonyl accumulation and loss of sulfhydryl groups) (Reznick et al. Biochem J 1992; 286: 607-11). CS contains both saturated and unsaturated aldehydes. The contribution of these aldehydes to the damaging effects of CS on human plasma was investigated. Aldehydes present in CS did not cause a depletion of plasma antioxidants such as ascorbic acid or alpha-tocopherol and did not induce plasma lipid peroxidation. Aldehydes decreased plasma protein sulfhydryl concentrations but increased protein carbonyls. The thiols glutathione and dihydrolipoic acid had a significant effect in reducing aldehyde-induced protein modifications.