Validate or falsify: Lessons learned from a microscopy method claimed to be useful for detecting<i>Borrelia</i>and<i>Babesia</i>organisms in human blood

Abstract

The structures interpreted as Borrelia and Babesia by the LM-method could not be verified by PCR. The method was, thus, falsified. This study underlines the importance of doing proper test validation before new or modified assays are introduced.