Journal of the American Veterinary Medical Association · 1971 · 65 citations · 1 references
Veterinary MedicineInfectious DiseasesVeterinary ParasitologyDiagnostic ParasitologyEnzymologyVeterinary Parasitic DiseasesParasite MorphologyParasite Staining TechniquesEnzyme ActivityHistochemical MarkersHistochemical MicroscopyPhosphatase Enzyme ActivityPhosphatase LocalizationPhosphatases
SUMMARY Acid phosphatase activity was demonstrated in smears of canine microfilariae by the naphthol AS-TR-phosphate method. Dirofilaria immitis and Dipetalonema reconditum were distinctly and accurately distinguished by this technique. Enzyme activity was restricted to two distinct zones in microfilariae of D. immitis , whereas in Dip. reconditum , acid phosphatase activity was uniformly distributed throughout the organism.
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