Microbiology and Immunology · 2016 · 29 citations · 37 references
Life SciencesMedicinal ChemistryMicrobiologyAntimicrobial PharmacologyMedical MicrobiologyMicrobial PathogenesisAntibacterial PharmacologyBacterial BiofilmsBacterial PathogenesisBacterial Virulence FactorsBiofilm FormationMicrobial BiofilmsAnti-biofilm StrategiesBacterial Biofilm AccumulationBacterial Biofilm EffectsBiofilm DisruptionBiofilm InhibitionBiofilm InhibitorsBiofilm-disrupting AgentsInhibitors Of Biofilm Formation
A novel benzimidazole molecule that was identified in a small-molecule screen and is known as antibiofilm compound 1 (ABC-1) has been found to prevent bacterial biofilm formation by multiple bacterial pathogens, including Staphylococcus aureus, without affecting bacterial growth. Here, the biofilm inhibiting ability of 156 μM ABC-1 was tested in various biofilm-forming strains of S. aureus. It was demonstrated that ABC-1 inhibits biofilm formation by these strains at micromolar concentrations regardless of the strains' dependence on Polysaccharide Intercellular Adhesin (PIA), cell wall-associated protein dependent or cell wall- associated extracellular DNA (eDNA). Of note, ABC-1 treatment primarily inhibited Protein A (SpA) expression in all strains tested. spa gene disruption showed decreased biofilm formation; however, the mutants still produced more biofilm than ABC-1 treated strains, implying that ABC-1 affects not only SpA but also other factors. Indeed, ABC-1 also attenuated the accumulation of PIA and eDNA on cell surface. Our results suggest that ABC-1 has pleotropic effects on several biofilm components and thus inhibits biofilm formation by S. aureus.
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The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage
Barry N. Kreiswirth, Sven Löfdahl, M J Betley et al. · Nature · 1983 · 1.3K citations