A method for the determination of nucleotide sequence in polyribonucleotides

Abstract

Our knowledge of the structure of ribonucleic acids (RNA) has advanced considerably in recent years. In the first place, the refinement of chromato- graphic techniques has made possible an accurate estimation of the purine and pyrimidine composi- tion (Vischer & Chargaff, 1948; Markham & Smith, 1949; Carter, 1950; Markham & Smith, 1951). Secondly, with the aid of both chemical and en- zymic methods it has been shown that the consti- tuent nucleosides in RNA are linked together as in I, -O.CH,< \Base P0 O.CH >Base