1c:I[419806,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/30036bedc5269936.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/cd5e572bc3e4eb92.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/d4ac9dcf27f6ff52.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 1d:T48c,THE MINIMAL adult requirement for folic acid is estimated to be 50μg per day.^1,2It is generally believed that requirements for folic acid are increased during pregnancy³; however, carefully documented data are lacking. In previous studies which measured the response to folic acid therapy in megaloblastic anemia of pregnancy,^4-7the folate content of diets was not quantitated and usually "titrated"⁷doses of folic acid were not administered. Recently investigations were initiated to study the requirements of folic acid during pregnancy. Some of the preliminary observations are described.

Methods

Laboratory Procedures.

—Standard hematologic tests, including the preparation of bone marrow for examination, were performed with established techniques.^8-10The quantity of vitamin B₁₂in serum was assayed withEuglena gracilis.¹¹All assays of folate activity were performed withLactobacillus casei. A modification¹²of the method of Herbert¹³was used for measuring serum folate activity. Whole-blood folate activity was determined by

	Year	Citations

References