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Induction of Lung Cancer in Germfree, Specific-Pathogen-Free, and Infected Rats by <italic>N</italic>-Nitrosoheptamethyleneimine: Enhancement by Respiratory Infection<xref ref-type="fn" rid="FN2">2</xref>

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1972

Year

Abstract

The effect of chronic respiratory infection on the development of lung cancer induced by N-nitrosoheptamethyleneimine was tested in rats. The nitrosamine was administered in drinking water for 22 weeks to germfree and specific-pathogen-free (SPF) rats and to rats with chronic murine pneumonia. All animals were killed 2 weeks after the last treatment. The incidence of lung neoplasms was 17% in germfree males, 37% in SPF males, and 83% in infected males. An incidence of 90–100% was found among females in all 3 experimental groups. The results in males demonstrate that chronic respiratory infection can enhance the neoplastic response of the lungs to a systemic carcinogen. The normal microbial flora does not seem to be essential for tumor induction by this nitrosamine. There was no significant difference in incidence of esophageal tumors among the 3 groups.—J Natl Cancer Inst 49: 1107–1114, 1972.