PubMed · 1992 · 10 citations · 0 references
Life SciencesImmunologyMolecular BiologyMolecular VirologyVaccinologyVirologyAnimal VirusesVeterinary VirologyViral InfectionsVirus PurificationAnimal Viral PathogenesisCdna CloningCdna Library ConstructionCdna SynthesisExperimental Animal Models Of Viral InfectionExpression Vector CloningFoot-and-mouth Disease In PigsVector Antigen Expression
A 0.9 kb double stranded cDNA of foot and mouth disease virus (FMDV) Type Asia 1, 63/72 was cloned in an expression vector, pUR222. A protein of 38 kd was produced by the clone which reacted with the antibodies raised against the virus. A 20 kd protein which may be derived from the 38 kd protein contained the antigenic epitopes of the protein VP1 of the virus. Injection of 10-20 micrograms of the partially purified 38 and 20 kd proteins or a lysate of cells containing 240 micrograms of the proteins elicited high titers of FMDV specific antibodies in guinea pigs and cattle respectively. Also, at these concentrations, the proteins protected 5 of 8 guinea pigs and 3 of 8 cattle when challenged with a virulent virus.