Illegitimate recombination occurs between the replication origin of the plasmid pC194 and a progressing replication fork.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

Allan M. Maxam, Walter Gilbert · Methods in enzymology on CD-ROM/Methods in enzymology · 1980 · 14.7K citations

Pharmacy, Biotechnology, Molecular Biology +13

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Raymond L. Rodriguez, Herbert L. Heyneker, Jorge H. Crosa et al. · PubMed · 1977 · 2.8K citations

Life Sciences, Genetics, Microbiology +17

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

Manuel Dagert, S. Dusko Ehrlich · Gene · 1979 · 1.5K citations

Life Sciences, Microbiology, Bacteriology +3

pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long

J. Gregor Sutcliffe · Nucleic Acids Research · 1978 · 760 citations · Full text

Life Sciences, Genetics, Immunology +14