Tissue fractionation studies. 7. Release of bound hydrolases by means of triton X-100

Abstract

Recent studies have disclosed the probable ex- istence of an intermediate class of cytoplasmic granules, apparently devoid of mitochondrial or microsomal enzymes, but containing several acid hydrolases, namely acid phosphatase, ribonuclease, deoxyribonuclease, cathepsin and f-glucuronidase (Appelmans, Wattiaux & Duve, 1955; Duve et al. 1955). Tentatively termed 'lysoscmes', these particles have been shown to behave as essentially inert little bags which, when ruptured, release their enzyme content in soluble and fully active form.