ChIP-seq reveals broad roles of SARD1 and CBP60g in regulating plant immunity

TLDR

Pathogen recognition triggers rapid transcriptional reprogramming and defense activation, yet the transcriptional control mechanisms of defense regulators remain largely unknown. The study aims to demonstrate that the transcription factors SARD1 and CBP60g bind to promoters of numerous plant immunity regulators. Chromatin immunoprecipitation sequencing was employed to map these transcription factor binding sites. The ChIP‑seq data reveal that SARD1 and CBP60g target both positive regulators of systemic, effector‑triggered, and PAMP‑triggered immunity and negative regulators, indicating that they act as master regulators of plant immune responses.

Abstract

Abstract Recognition of pathogens by host plants leads to rapid transcriptional reprogramming and activation of defence responses. The expression of many defence regulators is induced in this process, but the mechanisms of how they are controlled transcriptionally are largely unknown. Here we use chromatin immunoprecipitation sequencing to show that the transcription factors SARD1 and CBP60g bind to the promoter regions of a large number of genes encoding key regulators of plant immunity. Among them are positive regulators of systemic immunity and signalling components for effector-triggered immunity and PAMP-triggered immunity, which is consistent with the critical roles of SARD1 and CBP60g in these processes. In addition, SARD1 and CBP60g target a number of genes encoding negative regulators of plant immunity, suggesting that they are also involved in negative feedback regulation of defence responses. Based on these findings we propose that SARD1 and CBP60g function as master regulators of plant immune responses.