Diversity and function of plant growth promoting Rhizobacteria associated with wheat Rhizosphere in North Himalayan Region

Abstract

Little is known about the composition of bacterial diversity associated with plant roots. The purpose of this study was to investigate the diversity of bacteria associated with the root of wheat. Present study revels, the diversity of bacterial isolates from soils under wheat cultivation in district Uttarkashi. Phenotypic and physiological characteristics of the isolates were record to categorize and identify the bacteria. In all 133 different bacteria isolates were recovered from four different locations of which two were rainfed and two were irrigated. The spread plate technique on nutrient agar was use to isolates and purifies all the strains. The characteristics of the bacterial strains were determined using the colony morphology, gram staining as well as biochemical properties. On the basis of biochemical characterization 44% were Bacillus sp. and 24% belong to Pseudomonas sp. Genera identified in the rhizosphere isolates were also found in the rhizoplane isolates. Shannon Winner index of microbial diversity was 1.752 in irrigated crop and 1.594 in rain fed crop but there were no significant differences.