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The Role of Acidic Proteins from Cytoplasmic Fractions of Krebs II Ascites Cells for Efficient Translation
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1979
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Molecular BiologyProtein SynthesisAcidic ProteinsAffinity ChromatographyProtein FoldingCytoplasmic FractionsProteomicsProtein FunctionBiochemistryRna Structure PredictionProtein TransportCell BiologyStructural BiologyProtein BiosynthesisProtein PhosphorylationEfficient TranslationStepwise ElutionNatural SciencesCellular BiochemistryMedicine
Acidic proteins with affinity to RNA from cytoplasmic fraction of Krebs II Ascites cells were isolated by means of affinity chromatography on RNA-Sepharose CN-Br-columns. Stepwise elution with 350 mM [K+] and 1000 mM [K+] removed two fractions of proteins both of which are required for the formation of 40S- or 80S-initiation complexes and for efficient translation.