Publication | Open Access
Comparison of Procedures for Estimating Viability of Prunus Pollen
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1989
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BiologyPlant BiologyAllergyBotanyAgar-plate GerminationNatural SciencesPlant ReproductionPrunus PollenPlant PathologyMedicineVitro Germination TestsPlant PhysiologyPollen Viability
Abstract Seven assays (hanging-drop slide and agar-plate germination, acetocarmine, three tetrazolium-based stains, and Alexander’s staining procedures) were used to estimate pollen viability in Prunus armeniaca L., P. avium L., P. dulcis Webb, P. persica (L.) Batsch, and P. salicina Lindl. The two in vitro germination tests (hanging-drop and agar-plate) were the most reliable and were highly correlated ( r = 0.96). The pollen staining procedures were not reliable or consistent and were not positively correlated with the in vitro assays. Acetocarmine and Alexander’s stains stained dead pollen.