Evaluation of a glucose oxidase-peroxidase method adapted to the single-channel autoanalyzer and SMA 12-60.

Abstract

The glucose oxidase-peroxidase method for deter-mining serum glucose, with the ammonium salt of 2,2’-azine-di- (3- ethyl- benzothiazoline-(6)-sulfonic acid) as the chromogen, was adapted to use with the single-channel AutoAnalyzer and the SMA 12/60 (Technicon Corp.). The extended linearity (up to 500 mg/100 ml on both systems) eliminated the need for numerous dilutions in the 300 to 500 mg/100 ml range as required by other methods of analysis. Re-covery of added glucose was excellent. Supranormal concentrations of uric acid and ascorbic acid inter-fered with recovery, but the effects of elevated biliru-bin, creatinine, and urea, and of gross hemolysis were negligible. Sera of 337 patients (glucose con-centrations: 50 to 454 mg/100 ml) were analyzed by