Human Reproduction · 1987 · 252 citations · 0 references
Veterinary MedicineCell BiologyDevelopmental BiologyAssisted Reproductive TechnologiesIn Vitro FertilizationReproductive MedicineCell CryopreservationEmbryo Viability AssessmentOocyte CryobiologyOocyte CryopreservationThawing ProceduresFertilizationHuman Oocyte CryopreservationOocyte Survival RateOocyte ThawingPolyploidyPost-thaw Fertilization RatePost-thaw Fertilizing AbilityReproductive Outcomes After Cryopreservation
Three different methods were used for freezing human excess oocytes (320 frozen, 205 thawed) in our IVF programme and the results of these methods were compared. A high fertilization rate (75%) could be achieved after thawing, using 1,2 propanediol as a cryoprotectant. Polyploidy rates of 20% and 40% were observed using DMSO and 1,2-propanediol as cryoprotectants, respectively. Using the ultracooling method, the survival rate was poor (4%).